Micron Optics - Nikon Microscope Systems for New Jersey and Puerto Rico

How to segment and measure Channels with Consistent numbering:

1)      Channel 1 + Channel 2

2)      Channel 1

3)      Channel 2

4)      Background

5)      Channel 1 – Background

1.         Channel 1 + Channel 2

Segment objects using a multi or any single channel image.  In example below, 2-Channel Image was segmented using the Blue channel.  Cell #1 is highlighted in magenta and has an average grey scale value of 144.47.  A binary image (and cell numbering mask) is automatically generated with the measurement and is saved by the menu command REFERENCE > Binary -> Reference binary.

2.         Channel 1 (Blue)

The cell numbering g binary mask is applied to the Blue channel by selecting the blue channel and using the menu command REFERENCE > Binary <- Reference binary. Note the average value for cell #1 is 191.19.

3.         Channel 2 (Red)

The cell numbering g binary mask is applied to the Red channel image by selecting the image Red Channel image and using the menu command REFERENCE > Binary <- Reference binary. Note the average value for cell #1 is about 97.  Note that the value 144 is the average of the values 191 and 97.

4.         Background Measurement

Using a region of interest ROI tool select an area of the background.  In the example below, the average background is 8.03.

Micron Optics, 240 Cedar Knolls Road , Cedar Knolls, NJ 07927

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