Micron Optics
- Nikon Microscope Systems for New Jersey and Puerto Rico
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Special Techniques using NIS-Elements Manually separate touching objects Enclose objects that are not touching Remove objects from one channel only Adjust LUTs for individual objects in a field Measure objects in different channels with consistent numbering manually separate touching objects...
1. Open Image and object count window 2. Count objects by adjusting threshold, clean, smoothing, and restrictions 3. Depress “Keep updating count” button (on far right side of object count window) 4. Press TAB key to enter Bin nary editor 5. If desired, reduce/increase the transparency of the binary image with “CTRL-down (or up) arrow. 6. Select 6-Pt pen line width (near top of screen) 7. Toggle “FG” (foreground) to “BG” (Background) in Binary editor 8. Select Polyline tool (Left toolbar) 9. Left click and hold, draw clear line between touching objects, right click to finish. 10. Exit Binary Editor
To enclose objects that are not touching…
Repeat steps 1-7 above
7. Select Polyline Fill Tool (from left toolbar) 8. Left click, draw enclosing circle, right click to finish 9. Exit Binary editor, count is automatically updated.
Remove Objects from one channel of a Multi-Channel image... This technique was successfully used to remove a large diffuse red blotch from a FISH cell while retaining the distinct red dots. REMOVE RED OBJECTS FROM WITHIN INDIVIDUAL OBJECTS 1. extract red channel top work on 2. reference…current image>reference (to activate image Arithmetics) 3. Binary...Binary editor (or tab key) 4. Polygon fill tool (F4) Circle object to keep 5. Exit Binary editor 6. Reference…Image Arithmetics…Current AND Binary 7. Uncheck “View Overlay” Button
RETURN TO ORIGINAL IMAGE 8. Remove red channel 9. drag modified red channel back onto original image
Adjust LUTs for individual objects in a field... 1. Open Fresh Image 2. reference…current image>reference (to activate image Arithmetics) 3. Binary...Binary editor (or tab key) 4. Polygon fill tool (F4) Circle object to keep 5. Exit Binary editor 6. Reference…Image Arithmetics…Current AND Binary
Delete unwanted objects such as dirt and debris from around other objects in brightfield... 1. Fresh Image 2. reference…current image>reference (to activate image Arithmetics) 3. Binary...Binary editor (or tab key) 4. Polygon fill tool (F4) Circle object to keep 5. Exit Binary editor 6. Reference…Image Arithmetics…Current AND Binary
To Remove NIS-Elements when the Uninstall shield doesn't work... 1. Delete the NIS-Elements Folder 2. Run the Clean_Util.zip program 3. Delete the following folder... C:\Program Files\InstallShield Installation Information\{CD229881-9012-4B72-9FF7-6719C44B5CB0} (InstallSheild Installation is a hidden folder so make sure you have the option to see hidden folders on).
Micron Optics, 240 Cedar Knolls Road , Cedar Knolls, NJ 07927 973-267-5799 phone 973-267-6598 fax
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